近年来, 许多研究人员不断努力为药物、除草剂、食品添加剂等小分子物质高特异性、高灵敏的检测和分析开发新的方法和技术。然而, 目前通用的分子检测方法的实施需要较长的前处理时间、昂贵的大型仪器设备及专业操作人员, 无法实现有选择的识别及快速的现场检测。所以, 在本研究中我们将量子点表面分子印迹聚合物(QDs@MIPs)与光纤相结合, 构建了一种新的光纤探头, 并将该光纤探头应用于光纤传感器, 检测小分子物质莱克多巴胺(RAC)。试验中, 我们对QDs@MIPs的表征、光纤探头的性能、光纤探头对RAC的浓度响应、光纤传感器的特异性及光强分布进行了探究。研究结果表明, 该光纤探头应用于光纤传感器能够提高光纤传感器的灵敏度, 使分子印迹光纤传感器具有更高的特异性识别能力和较强的抗干扰能力, 同时检测过程简便快捷, 适用于快速的现场检测。

Soluble microneedles (MNs) have recently become an efficient and minimally invasive tool in transdermal drug delivery because of their excellent biocompatibility and rapid dissolution. However, direct monitoring of structural and functional changes of MNs in vivo to estimate the efficiency of insulin delivery is difficult. We monitored the dissolution of MNs to obtain structural imaging of MNs' changes by using optical coherence tomography (OCT). We also observed the effect of MNs on microvascular conditions with laser speckle contrast imaging (LSCI) and measured the blood perfusion of skin to obtain functional imaging of MNs. We determined the performance of two soluble MN arrays made from polyvinyl alcohol (PVA) and polyvinyl alcohol/ polyvinylpyrolidone (PVA/PVP) by calculating the cross-sectional areas of the microchannels in mouse skin as a function of time. Moreover, the change in blood glucose before and after using MNs loaded with insulin was evaluated as an auxiliary means to demonstrate the ability of the soluble MNs to deliver insulin. Results showed that the structural imaging of these MNs could be observed in vivo via OCT in real time and the functional imaging of MNs could be showed using LSCI. OCT and LSCI are potential tools in monitoring MNs structural and functional changes.